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polyacrylamide hydrogel substrates  (Thermo Fisher)


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    Thermo Fisher polyacrylamide hydrogel substrates
    Polyacrylamide Hydrogel Substrates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyacrylamide+hydrogel+substrates/polyacrylamide+hydrogel+substrates/pm38811770-285-10-27
    Average 90 stars, based on 1 article reviews
    polyacrylamide hydrogel substrates - by Bioz Stars, 2026-10
    90/100 stars

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    Microscopy:

    Article Title: Vimentin is a key regulator of cell mechanosensing through opposite actions on actomyosin and microtubule networks.
    Article Snippet: .. Cell traction force microscopy To perform traction force microscopy experiments, polyacrylamide hydrogel substrates of desired stiffness with 1% of 200 nm fluorescently labeled green beads (2% solid, Thermo Fisher Scientific) were prepared as described in ref. 89. ..

    Labeling:

    Article Title: Vimentin is a key regulator of cell mechanosensing through opposite actions on actomyosin and microtubule networks.
    Article Snippet: .. Cell traction force microscopy To perform traction force microscopy experiments, polyacrylamide hydrogel substrates of desired stiffness with 1% of 200 nm fluorescently labeled green beads (2% solid, Thermo Fisher Scientific) were prepared as described in ref. 89. ..



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    The PC9 cells was seeded on 5-kPa <t>PAA</t> gel and imaged for over 10 hours after attaching. ( A ) YAP expression is represented by green fluorescence intensity. Note: The YAP intensity becomes homogenous from the 1.5 th hour on. (B) Substrate deformation (overlapped with the bright-field image) at cell location is represented <t>by</t> <t>fluorescent</t> beads displacement field at each time point. Displacement direction and magnitude are shown by the arrow direction and color, respectively. The displacement field caused by PC9 cells is smaller compared to that caused by the B2B cell. Throughout the 10-hour spreading process, the area of PC9 cells nearly remains constant. (C) Traction field (overlapped with the bright-field image) calculated from displacement field. The traction generated by this representative PC9 cell gradually decreases from the 6 th hour to the 10 th hour.
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    The PC9 cells was seeded on 5-kPa <t>PAA</t> gel and imaged for over 10 hours after attaching. ( A ) YAP expression is represented by green fluorescence intensity. Note: The YAP intensity becomes homogenous from the 1.5 th hour on. (B) Substrate deformation (overlapped with the bright-field image) at cell location is represented <t>by</t> <t>fluorescent</t> beads displacement field at each time point. Displacement direction and magnitude are shown by the arrow direction and color, respectively. The displacement field caused by PC9 cells is smaller compared to that caused by the B2B cell. Throughout the 10-hour spreading process, the area of PC9 cells nearly remains constant. (C) Traction field (overlapped with the bright-field image) calculated from displacement field. The traction generated by this representative PC9 cell gradually decreases from the 6 th hour to the 10 th hour.
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    The PC9 cells was seeded on 5-kPa PAA gel and imaged for over 10 hours after attaching. ( A ) YAP expression is represented by green fluorescence intensity. Note: The YAP intensity becomes homogenous from the 1.5 th hour on. (B) Substrate deformation (overlapped with the bright-field image) at cell location is represented by fluorescent beads displacement field at each time point. Displacement direction and magnitude are shown by the arrow direction and color, respectively. The displacement field caused by PC9 cells is smaller compared to that caused by the B2B cell. Throughout the 10-hour spreading process, the area of PC9 cells nearly remains constant. (C) Traction field (overlapped with the bright-field image) calculated from displacement field. The traction generated by this representative PC9 cell gradually decreases from the 6 th hour to the 10 th hour.

    Journal: bioRxiv

    Article Title: All-optical mechanobiology interrogation of YAP in human cancer and normal cells using a novel multi-functional system

    doi: 10.1101/2021.06.09.447829

    Figure Lengend Snippet: The PC9 cells was seeded on 5-kPa PAA gel and imaged for over 10 hours after attaching. ( A ) YAP expression is represented by green fluorescence intensity. Note: The YAP intensity becomes homogenous from the 1.5 th hour on. (B) Substrate deformation (overlapped with the bright-field image) at cell location is represented by fluorescent beads displacement field at each time point. Displacement direction and magnitude are shown by the arrow direction and color, respectively. The displacement field caused by PC9 cells is smaller compared to that caused by the B2B cell. Throughout the 10-hour spreading process, the area of PC9 cells nearly remains constant. (C) Traction field (overlapped with the bright-field image) calculated from displacement field. The traction generated by this representative PC9 cell gradually decreases from the 6 th hour to the 10 th hour.

    Article Snippet: Polyacrylamide (PAA) hydrogel substrates (with 2 % of fluorescent beads (Invitrogen)) that have Elastic Modulus 2 kPa, 5 kPa, 10 kPa, and 40 kPa are prepared following the established protocols [ ], [ ].

    Techniques: Expressing, Fluorescence, Generated